Nowadays, the use of plate girder in different kinds of structures has been increased. One of these is plate girder with delta stiffener. Investigations have shown that the bending and shearing behavior of plate girder with delta stiffener are better than usual plate girder with uniform sections. Preparing web openings to make space for service, inspection and passing the big pipes is unavoidable in plate girders. In this study, employing ANSYS v.12 software, the modeling of hole in the web of plate girder was done and the effective parameters on shear buckling strength of perforated steel plate girder was considered to study the structural behavior and optimum range for Preparation of web hole and the best form of ordinary kinds of hole. The results show that, the best web performance location is between 0.30 until 0.35 of length, optimum of effective height parameter (ratio of hole diameter to distance of stiffener connection from intersection at web to the bottom flange) is between 0.26 until 0.27, and there is a linear behavior between shear buckling strength versus changes in hole diameter of opening which is presented by an equation. The comparison between different opening shape with equal surface (square, circle and triangle) indicates that the square opening has the priority in buckling shear strength in comparison with other shapes.